Sumo Vector

Sumo Vector. Includes The Champion pET SUMO TA Cloning box contains linearized Champion pET SUMO vector sterile water dNTPs 10X PCR Buffer control template and primers T4 DNA ligase 10X ligation buffer primers for sequencing or PCR screening and an expression control The SUMO Protease box contains SUMO Protease and buffers.

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Champion™ pET SUMO Expression System US

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PCR fragments were subcloned into pET246× HisSUMO or pET246× His (a parallel vector that does not carry the SUMO sequence) to produce parallel sets of constructs encoding 6× HisSUMO and 6× His fused versions of the proteins of interest All plasmids were routinely sequenced Table 1 PCR primers for amplifying the SARSCoV protein genes Proteins Region.

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PDF fileThe following reagents are included with the pET SUMO vector (Box 1) Note that the user must supply Taq polymerase Store Box 1 at 20°C Item Concentration Amount pET SUMO vector linearized 25 ng/ μl in 10 mM TrisHCl pH 80 1 mM EDTA pH 80 5 x 10 l 10X PCR Buffer 100 mM TrisHCl pH 83 (at 42°C) 500 mM KCl 25 mM MgCl 2 001% gelatin 100 μl dNTP Mix 125.